Searchable abstracts of presentations at key conferences in endocrinology

ea0037ep223 | Calcium and Vitamin D metabolism | ECE2015

Development of an ELISA for the measurement of free 25OH vitamin D

Heureux Nicolas , Anciaux Michel , Poncelet Martin , Mathieu Fabienne , Swinkels Leon , Huijs Tonnie , Boerrigter Saskia , Lindhout Ernst , Mayer Gregg , Martens Mike

The objective of this project was to develop and characterise a simple immunoassay for the quantification of free 25-hydroxyvitamin D (25OH Vit D). Recent studies suggest that the concentration and genotype of vitamin D binding protein (DBP) are important factors that determine the bioavailability of 25OH Vit D in blood. It has been suggested that measurement of free, non-protein bound 25OH Vit D in serum, may provide more relevant diagnostic information than total 25OH Vit D,...